Extravagant eating and drinking, and abuse of power, are also formally banned, said Xinhua news agency.
The party has in the past warned its officials to refrain from extravagant dinners and purchasing mooncakes using public funds.
China has been conducting a strict anti-corruption drive since 2012.
The new rule on golf states that members are banned from "obtaining, holding or using membership cards for gyms, clubs, golf clubs, or various other types of consumer cards, or entering private clubs".
If caught, members could either receive a warning or be removed from the party, depending on the severity of the violation.
The new regulations did not explain why the joining of golf clubs is banned, but such clubs are often seen by the Chinese public as places where officials have cut shady deals.
Another new rule states they cannot "violate official provisions on hospitality management and engage in over-the-top entertaining, or take advantage of opportunities for extravagant eating and drinking".
The Communist Party has also rephrased a previous clause banning adultery and mistresses, which now says that members are banned from "having improper sexual relations with other people which have bad repercussions".
The party`s strict rules for officials has in the past affected business for restaurants and luxury goods retailers in China and elsewhere.
President Xi Jinping has led a major anti-corruption campaign since taking office three years ago.
He has previously warned of unrest if corruption and perceived privilege within the Communist Party are not tackled.
China golf: Communist Party bans club membership
22 October 2015
Read: 2010
The Chinese Communist Party has banned all 88 million of its members from joining golf clubs, in its latest update of party discipline rules.
